By I. Scharrer (auth.), Professor Dr. med. Inge Scharrer, Professor Dr. med. Wolfgang Schramm (eds.)

This booklet comprises the contribution to the thirty seventh Hemophilia Symposium, Hamburg 2006. the most themes are epidemiolgy, remedy of inhibitors in hemophiliacs, hemophilic arthropathy and synovitis, suitable hemophilia remedy 2006, and pediatric hemostasiology. the amount is rounded off by means of quite a few loose papers and posters on hemophilia, casuistics, and diagnostics.

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Blood was taken and analyzed before vaccination and at several time points after vaccination. Table 2. Characteristics of healthy volunteers included in the study Healthy volunteers Age (in years) Last booster with Tetanus vaccine V01 39 2005 V02 28 2000 V03 49 2000 V04 29 2002 V05 36 1989 V06 31 1996 V07 25 1989 V08 36 2005 V09 30 2002 V10 27 unknown V11 43 1999 V12 36 1999 Blood Sampling and Cell Preparation Blood was collected and peripheral blood mononuclear cells (PBMC) were prepared using Vacutainer cell preparation tubes (CPT) with sodium citrate (Becton Dickinson, Schwechat, Austria).

AIDS. 2004 Feb 20;18(3):525–33. 15 16 W. Schramm and H. Krebs 3. 4. 5. 6. 7. Quintana M, del Amo J, Barrasa A, Perez-Hoyos S, Ferreros I, Hernandez F, Villar A, Jimenez V, Bolumar F: Progression of HIV infection and mortality by hepatitis C infection in patients with haemophilia over 20 years. Haemophilia. 2003 Sep;9(5):605–12 Ragni MV, Belle SH: Impact of human immunodeficiency virus infection on progression to end-stage liver disease in individuals with hemophilia and hepatitis C virus infection.

Recently, Crotty et al. [7] developed an assay that allows the quantification of antigen-specific memory B cells in human blood. This assay uses a 6-day polyclonal stimulation of PBMCs followed by an antigen-specific ELISPOT for the detection of memory B cells that have differentiated into antibody-secreting plasma cells in vitro. We adapted this human memory B cell assay to human FVIII and used it to track FVIII-specific memory B cells in the blood of patients with and without FVIII inhibitors. In order to validate the assay, we studied tetanusspecific memory B cells and tetanus-specific antibody-producing plasma cells in healthy volunteers.

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