By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This handbook presents technical protocols for musculoskeletal examine on a translational foundation, i.e. a disease-orientated method. It deals information on a variety of laboratory innovations, together with phone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal types, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so forth. The content material is easy and simple, with illustrations and step by step approaches as a simple experimental reference for body of workers in simple and medical musculoskeletal study and schooling. This e-book will offer a special multidisciplinary platform for numerous professions -- not just orthopedics, but additionally biomedical engineering and biomaterial sciences -- concerning either uncomplicated and medical medication.
Contents: phone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal types; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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Extra resources for A Practical Manual for Musculoskeletal Research
Com www. com www. com www. com www. com www. com www. com www. com www. com www. com www. com www. com www. com www. com www. gov www. org/tdb/microarray www. com Note: This list of suppliers is far from complete, but should provide the reader with a few options enabling familiarization with some of the tools available. (bioinformatics). We believe, on the basis of experience acquired by us and other investigators to date, that the technology of gene array analysis can make significant contributions to musculoskeletal research.
Test and reference RNA samples are then reverse-transcribed and labeled with different fluorodyes (Cy5 and Cy3), which fluorescence in different (red and green, respectively) wavelength bands; these are hybridized to the microarray. The fluorescence of each dye is then measured for each feature (gene) using laser excitation, and converted to relative expression levels in the two samples. in the test and reference samples (Starkey 2001; Du et al. 2006; Zheng et al. 2005). The most widely used method of array fabrication is the robotic spotting of individual DNA clones onto a coated glass slide.
Qxd 8/12/2008 12:21 PM Page xxxix List of Contributors xxxix Ting-Ting Tang Department of Orthopaedic Surgery Shanghai Ninth People’s Hospital Shanghai Jiao Tong University School of Medicine, Shanghai P. R. China Hong-Fu Wang Department of Bone Metabolism Institute of Radiation Medicine Fudan University, Shanghai P. R. China James Hui-Cong Wang MechanoBiology Laboratory Department of Orthopaedic Surgery Department of Bioengineering Department of Mechanical Engineering Department of Materials Science and Engineering Department of Physical Medicine & Rehabilitation University of Pittsburgh Pittsburgh, PA 15213 USA Jing-Mei Wang Norland — a CooperSurgical Company Beijing 100032 P.