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00 h and few attacked at night. Both sexes of G. palpalis fuscipes attacked most commonly in the middle of the day. Both sexes of G. brevipalpis attacked most in the hours following sunrise and sunset but the species was taken at all hours of both day and night. The species most affected by weather conditions was G. pallidipes ; evening rain depressed or suppressed the evening activity wave. G. brevipalpis was most commonly caught during the day when the weather was overcast, or at shaded catching stations; it frequently showed a sudden wave of activity just before an afternoon or evening storm and this replaced the usual wave of activity near sunset.
Such differences might, however, have been determined in part by olfactory factors. Catchers were found to vary significantly in their performance and so experimental designs are required in which isolation of such variation is possible. Fly-round catches were similar in both wet and dry seasons but both baited and trap catches were markedly reduced in the latter, indicating that it should not be assumed that fly-round data give a reliable measure of the risk of being infected with trypanosomes.
THETRYPANOSOME A . T H E ESTABLISHMENT OF T H E I N F E C T I O N 1. Early Development The work of Gordon et al. (1956) indicated that the saliva of Glossina at the time of biting (and therefore the metacyclic forms of T. brucei subgroup trypanosomes) was likely to enter the capillary circulation as well as the tissue spaces. In further studies, these workers (Willett and Gordon, 1957; Gordon and Willett, 1958) have considered this result in relation to the establishment of T. brucei subgroup infections in man, monkeys, guinea-pigs, rabbits and rats.